Although eDNA was heterogeneously distributed in the environment, occupancy models were able to provide the first estimates of detection probabilities, which were greater than 91%. Burmese python eDNA was detected in 37 of 63 field sampling events however, the other species were not detected. We validated the python and boa constrictor assays using laboratory trials and tested all species in 21 field locations distributed in eight southern Florida regions. Burmese pythons, Northern African pythons, and boa constrictors are established and reproducing, while the green and yellow anaconda have the potential to become established. sebae), boa constrictor ( Boa constrictor), and the green ( Eunectes murinus) and yellow anaconda ( E. To assist with detection, we developed species-specific eDNA assays using quantitative PCR (qPCR) for the Burmese python ( Python molurus bivittatus), Northern African python ( P. Giant constrictor snakes pose a threat to native species and the ecological restoration of the Florida Everglades. Low detection of invasive giant constrictors using visual surveys and traps has hampered the estimation of occupancy and detection estimates needed for population management in southern Florida, USA. However, occupancy terminology has been applied inconsistently in eDNA studies, and many have calculated occurrence probabilities while not considering the effects of imperfect detection. Applied in eDNA studies, occupancy models can be used to estimate occurrence and detection probabilities and thereby account for imperfect detection.
Environmental DNA (eDNA) methods are used to detect DNA that is shed into the aquatic environment by cryptic or low density species.
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